Duo Spore Biological Indicator Test with Culture Service
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Duo Spore® Biological Indicators with Culture Media and Service - 12 test envelopes per order.
Mail in your spore test and get results in 48 hours.
OEM #26909400
These tests monitor sterilization processes in Steam Autoclaves, Ethylene Oxide (EtO) Sterilizers and Dry Heat (DH) Sterilizers.
The Duo-Spore® Biological Indicator Test with Culture Service is a reliable and compliant method for monitoring steam, ethylene oxide, and dry heat sterilization cycles in clinical and non-clinical settings. These test strips, prepared from Geobacillus stearothermophilus and Bacillus atrophaeus, come in a test envelope containing two test strips and one control strip in two color-separated pouches. The D-values and Survival/Kill performance data comply with U.S. Pharmacopeia requirements
Features:
Mail in your spore test and get results in 48 hours.
OEM #26909400
These tests monitor sterilization processes in Steam Autoclaves, Ethylene Oxide (EtO) Sterilizers and Dry Heat (DH) Sterilizers.
The Duo-Spore® Biological Indicator Test with Culture Service is a reliable and compliant method for monitoring steam, ethylene oxide, and dry heat sterilization cycles in clinical and non-clinical settings. These test strips, prepared from Geobacillus stearothermophilus and Bacillus atrophaeus, come in a test envelope containing two test strips and one control strip in two color-separated pouches. The D-values and Survival/Kill performance data comply with U.S. Pharmacopeia requirements
Features:
- Biological indicator test for sterilization monitoring.
- 12 test envelopes with control strips.
- Indicator strips with Geobacillus stearothermophilus and Bacillus atrophaeus.
- Convenient mail-in culture service program.
- Suitable for steam, ethylene oxide and dry heat sterilization.
- Lab technicians process results in 48 hours.
- Dual monitoring for clinical and non-clinical settings.
- Glassine pouch allows worry-free transfer.
- Quick and accurate results for verification.
Technical Specifications
| Process | Steam, EO Gas and Dry Heat |
|---|---|
| Packaging | 12 tests |
| Includes | 12 biological indicators, 12 envelopes and cultural service results |
| Microorganisms | Geobacillus stearothermophilus and Bacillus atrophaeus |
| Test Envelope | Two test strips and one control strip in two color-separated pouches |
| Results | Lab technicians process results in 48 hours |
| OEM | 26909400 |
FAQ
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Scroll down for frequently asked questions
Explore Our FAQs for Helpful Answers
Find answers to common questions about this product, including features, specifications, and operation. Need more help? Our team is here to assist.
How often should I spore test my sterilizer?
How often should I spore test my sterilizer?
The CDC recommends biological monitoring (spore testing) of each sterilizer at least weekly, and with every load that contains an implantable device. Your state or local regulations may require more frequent testing.
What should I do if a spore test fails (positive result)?
What should I do if a spore test fails (positive result)?
Take the sterilizer out of service and repeat the spore test right away using the same cycle. If the mechanical and chemical indicators show the sterilizer is working properly, a single positive result often points to operator error, such as loading or packaging. If the repeat test is also positive, do not use the sterilizer until it has been inspected or repaired and passes spore tests in three consecutive cycles.
Isn't the color change on the pouch or tape enough?
Isn't the color change on the pouch or tape enough?
No. Chemical indicators show that the sterilizing agent reached the item, but they do not prove sterilization was achieved. Only a biological indicator (spore test) confirms that highly resistant spores were killed.
Mail-in or in-office spore testing: which is better?
Mail-in or in-office spore testing: which is better?
Both are accepted. Mail-in services do the incubation and recordkeeping for you, but results depend on mail transit time. In-office systems give results in 24 to 48 hours, or minutes with rapid-readout indicators, but your staff must incubate and keep the records.
Which sterilizers can the Duo-Spore test monitor?
Which sterilizers can the Duo-Spore test monitor?
Steam, ethylene oxide (EtO) and dry heat sterilizers.
How does the mail-in culture service work?
How does the mail-in culture service work?
Run the test strips through your sterilizer, then mail them in. Lab technicians process the results within 48 hours after the indicators arrive at the lab.
What is in each test envelope?
What is in each test envelope?
Two test strips and one control strip in two color-separated pouches.
Which spores are on the strips?
Which spores are on the strips?
Geobacillus stearothermophilus and Bacillus atrophaeus.
How many tests are in this order?
How many tests are in this order?
12 test envelopes, each with control strips, plus the culture service results.

